The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein.
نویسندگان
چکیده
Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein-oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.
منابع مشابه
Activator Generated Electron Transfer Combined Atom Transfer Radical Polymerization (AGET-ATRP) for Controlled Grafting Location of Glycidyl Methacrylate on Regenerated Cellulose Ultrafiltration Membranes
This investigation indicates the ability to selectively graft glycidyl methacrylate (GMA) only from the external surface of regenerated cellulose (RC) ultrafiltration (UF) membranes using activator generated electron transfer (AGET) atom transfer radical polymerization (ATRP). This controlled polymerization resulted in epoxy functionalized polymer brush ends. Further reaction of the terminal ep...
متن کاملSynthesis and characterization of acrylic acid grafted gum tragacanth and its effect in Fe2+ removal
In this paper, grafting of acrylic acid monomer on Gum tragacanth was performed. Variable of experiment included concentration of initiators and monomer, time, and temperature. Grafting efficiency was measured in the term of percentage of grafting (%G) and percentage of grafting efficiency (%E). Effectiveness of grafted gum in ferrous ion removal at different condition was assessed. According t...
متن کاملاثر زمان و روش پیوند بر گیرایی و رشد پیوندک در گردوی ایرانی (Juglans regia L.)
This study was carried out to determine the best method and time of budding and grafting in walnut under controlled conditions in Saman, Chaharmahal Va Bakhtiari Province. The experiment was in completely randomized design with 13 treatments, including cleft, splice and whip-tongue grafting on February 4, chip budding on April 4, 19, May 5 and 20, and T-budding and patch budding each one on fir...
متن کاملNanometric grafting of poly (N-isopropylacrylamide) onto polystyrene film by different doses of gamma radiation
Poly N-isopropylacrylamide was successfully grafted onto a polystyrene cell culture dish by gamma ray. In this study, the effect of a radiation dose (radiation absorbed dosages of 10, 20, 30, 40 KGy) under appropriate temperature and grafting conditions was investigated. The FTIR analysis showed the existence of the graft PNIPAAm on the substrate. The optimal value of the dose for grafting was ...
متن کاملNanometric grafting of poly (N-isopropylacrylamide) onto polystyrene film by different doses of gamma radiation
Poly N-isopropylacrylamide was successfully grafted onto a polystyrene cell culture dish by gamma ray. In this study, the effect of a radiation dose (radiation absorbed dosages of 10, 20, 30, 40 KGy) under appropriate temperature and grafting conditions was investigated. The FTIR analysis showed the existence of the graft PNIPAAm on the substrate. The optimal value of the dose for grafting was ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 25 شماره
صفحات -
تاریخ انتشار 2015